Journal: European Journal of Microbiology & Immunology
Article Title: The conundrum of colonization resistance against Campylobacter reloaded: The gut microbota composition in conventional mice does not prevent from Campylobacter coli infection
doi: 10.1556/1886.2020.00004
Figure Lengend Snippet: Colonic secretion of pro- and anti-inflammatory mediators in Campylobacter infected conventional mice. Conventionally colonized C57BL/6 mice were perorally infected with C. jejuni (open circles) or C. coli (closed circles) on day (d) 0 and d1 by gavage. On day 21 post-infection, (A) IFN-γ, (B) TNF-α, (C) MCP-1, (D) IL-6, (E) IL-12p70 and (F) IL-10 concentrations were determined in supernatants derived from colonic ex vivo biopsies. Naive mice served as negative control animals (open diamonds). Medians (black bars), levels of significance (P -values) assessed by the Kruskal-Wallis test and Dunn’s post-correction and numbers of analyzed animals (in parentheses) are indicated. Data were pooled from four independent experiments
Article Snippet: Colonic ex vivo biopsies were cut longitudinally, washed in phosphate buffered saline (PBS; Gibco, Life Technologies, UK), and strips of approximately 1 cm 2 tissue and ex vivo biopsies derived from the spleen (one half) were placed in 24-flat-bottom well culture plates (Nunc, Germany) containing 500 μL serum-free RPMI 1640 medium (Gibco, life technologies, UK) supplemented with penicillin (100 U/mL) and streptomycin (100 μg/mL; PAA Laboratories, Germany).
Techniques: Infection, Derivative Assay, Ex Vivo, Negative Control